The fluorescence of etbr increases 21fold upon binding to doublestranded rna and 25fold on binding doublestranded dna so that destaining the background is not necessary with a low stain concentration 10. Us federal regulations potassium bromide 7758 02 3 listed on the united states tsca toxic substances control act inventory. To avoid the risk of static discharges and gas ignition, soak contaminated clothing thoroughly with water before removing it. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet.
Ethidium bromide intercalates inserts itself between nucleic acid base pairs and exhibits fluorescence under ultraviolet light, providing molecular biologists with an excellent stain for visualizing and imaging nucleic acids. Ethidium bromide 1% solution in water for electrophoresis find msds or sds, a coa, data sheets and more information. Ethidium bromide is commonly used to detect nucleic acids in molecular biology laboratories. Ethidium bromide should be handled in the laboratory using the basic prudent practices described in chapter 5. Specifications of ammonium bromide commercial pure, ip bp, usp, acs, ar, fcc food grade manufacturers. Ethidium bromide etbr is the most commonly used nucleic acid stain for page or agarose gel electrophoresis. Safety data sheet according to 29cfr19101200 and ghs rev. If the pesticide product enduse labeling contains hazard. Ethidium bromide solution material safety data sheet sdsmsds. Ethidium bromide etbr is commonly used as a nonradioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and in other methods of nucleic acid separation. Material safety data sheet precast agarose gel cassette with ethidium bromide version. Material safety data sheet ethidium bromide sc203735 hazard alert code key.
This chapter lists the cas registry number, chemical structure, chemical abstract ca index name, other chemical names, merck index number, chemicaldye class, synthesis procedure, molecular formula. September 29, 2009 information in this msds is from available published sources and is believed to be accurate. Etbr is a dark red, crystalline, nonvolatile solid, moderately soluble in water, which fluoresces. Ethidium bromide solution bioreagent, for molecular. Safety data sheet creation date 08jun2010 revision date 18jan2018 revision number 4 1. Acute toxicity oral ld50 ld50 oralrat1,503 2,177 mgkg inhalation lc50 lc50 inhalationrat1 h11. Procedure for cleanup of laboratory surfaces contaminated. Liquid ethidium bromide waste can be collected in the same container as the solid waste. Ethidium bromide solution 10 mgml catalog or product number. Mobility will likely be mobile in the environment due to its volatility.
Otherwise, aqueous solutions must be deactivated beforedisposal. Material safety data sheet ethidium bromide solution sdsmsds. Reagent replacement kit for pippin prep gel cassettes product numbers. Form of exposure permissible concentration basis bromomethane 74839 twa 1 ppm acgih c 20 ppm 80 mgm3 osha z1 twa 5 ppm.
Ethidium bromide ethidium bromide is an intercalating agent which resembles a dna base pair. The detection limit of dna bound to ethidium bromide is 0. Chemical product and company identification section 1. Hazardous by definition of hazard communication standard 29 cfr 1910. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of. Identification product name ethidium bromide, 1% solutionmolecular biology cat no. This products resides on a fisher scientific gsa or va contract. Material safety data sheet potassium bromide msds section 1. Material safety data sheet dimidium bromide msds section 1. Ethidium bromide safe handling, disposal, and emergency procedures. This information will support in employment of this product in a very safe and secured manner. It fluorescents a redorange color under ultraviolet uv light and with increased fluorescence when bound to doublestranded dna.
Ethidium bromide is an intercalating agent commonly used as a fluorescent tag nucleic acid stain in molecular biology laboratories for techniques such as agarose gel electrophoresis. Msds contains all of the information required by those regulations. Canadian ingredient disclosure list section 16 additional information msds creation date. Separated from strong oxidants and food and feedstuffs. Material safety data sheet according to regulation ec no. Safety data sheet according to federal register vol. Singlestranded rna can also be detected, since it usually folds back onto itself and thus provides local base pairing for the dye to intercalate. Potassium bromide safety data sheet according to federal register vol. Therefore, it is commonly used as nucleic acid fluorescent tag in various techniques of the life science field. Material safety data sheet potassium bromide, 10% wv.
Thermo scientific pierce ethidium bromide is available as a concentrated 10 mgml solution to accommodate most molecular biology applications for fluorescent dna visualization. Ammonium bromide sds ghs msds sheet, material safety data sheet. Ac205000, ac205010, ac205050, ac205, ac246900000, ac246900025 ac246900025, ac24690, ac246905000, s2553, s255500 synonyms. Identification of the substances mixture and of the company undertaking 1. Ethidium bromide 1% solutionmolecular biology, fisher. Ethidium bromide is an intercalating dye used in the visualization of dna and rna in electrophoresis gels and cesium chloride density gradients. Material safety data sheet section 1 copperii bromide 1 chemical product and company identification msds name. Remove person to fresh air and keep comfortable for breathing. Material causing immediate and serious toxic effects toxic. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet emergency telephone number chemtreco, inside the usa. Material safety data sheet potassium bromate msds section 1. Potassium bromide, 10% wv 6 section 16 other information msds creation date. Sds ethidium bromide page 6 of 8 hazardous decomposition products formed under fire conditions.
All ethidium bromide waste must be disposed of as directed in this. Fisher scientific 1 reagent lane fair lawn, nj 07410 for information, call. Ethidium bromide solution material safety data sheet sds msds. Npropyl bromide, stabilized material safety data sheet section 1 revision date. Ammonium bromide einecs ec no 2351838, xi, irritant, r3638 chemical. Ethidium bromide is commonly used in molecular biology laboratories for dna identification in electrophoresis. It is available as an aqueous solution containing 10 mgml ultrapure ethidium bromide and highpurity water. Ethidium bromide is a dna intercalator, inserting itself between the base pairs in the double helix. This msds has been prepared according to the hazard criteria of the controlled products regulations cpr and the msds contains all of the information required by the cpr. Ethidium bromide etbr is a mutagen, a suspected carcinogen and teratogen, and at high concentrations is irritating to the eyes, skin, mucous membranes and upper respiratory tract.
Pippin preptm precast agarose gel cassettes with buffer solution product numbers. Product name ethidium bromide, 1% solutionmolecular biology cat no. No producers of eb were reported in epas 1983 tscapp database cis, 1994. Bp210 synonyms no information available recommended use laboratory chemicals. Fisher scientific 1 reagent lane fair lawn, nj 07410. Ultrapure ethidium bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and caesium chloride cscl gradients. Liquid ethidium bromide waste with a concentration less than 1. Ethidium bromide etbr is a flat molecule that fits between adjacent base pairs intercalates in the dna double helix.
Due to its unique structure, it can easily intercalate into dna strand. Find msds or sds, a coa, data sheets and more information. Sigmaaldrich offers a number of ethidium bromide solution products. This policy provides general guidance to researchers and labs on how to work safely with ethidium bromide. Articles of butylmagnesium bromide are included as well. No warranty, express or implied, is made and labchem inc. Other companies are charging thousands of dollars to set up accounts and. It is sometimes the cause of health and safety concerns for workers charged with using it during the course of their work. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. After inactivation, the solution, devoid of ethidium bromide,can be poured down the drain with copious amounts of water. The toxic effects of etbr may be experienced if swallowed, inhaled, or absorbed through the skin. Ethidium bromide is a very common fluorescent intercalating agent used for visualization of nucleic acids. Acros organics bvba janssen pharmaceuticalaan 3a 2440 geel, belgium company identification. The destaining bags are very simple and cheap to use.
Disposal considerations waste disposal methods chemical waste generators must determine whether a discarded chemical is classified as a. Because of its mutagenicity, stock solutions of this compound should be prepared in a fume hood, and protective gloves should be worn at all times while handling this substance. Potassium bromide msds potassium bromide manufacturer india. Simply drop a destaining bag into your solution, periodically swirl it around a few times, and let it stand overnight. First aid measures as this product is a gas, refer to the inhalation section. Ethidium bromide laboratory spill and waste handling. A uv light can be used to determine complete inactivation. Section 2 chemical identification 02172009 revision. Ethidium bromide 3,8diamino5ethyl6 phenylphenanthridinium bromide is the classic dna stain. Ethidium bromide has mutagenic properties that may present a hazard if it is poured down the drain untreated or placed in the trash. Disposal, decontamination, and destruction procedure. Procedure for cleanup of laboratory surfaces contaminated with ethidium bromide.
Material safety data sheet ethidium bromide solution. Material safety data sheet pippin prep replacement reagent kits. A safe and convenient way to use ethidium bromide, while minimizing exposurerelated. Ethidium bromide is typically in powder or solution form and is soluble in water. Eb was reported by watkins 1952 to be prepared from 3. Ethidium bromide has uv absorbance maxima at 300 and 360 nm, and an emission maximum at 590 nm.
Ac205000, ac205010, ac205, ac246900000, ac24690, ac246905000, s2553, s255500 synonyms. Chemical product and company identification product name. Extreme high moderate low section 1 chemical product and company identification product name ethidium bromide statement of hazardous nature considered a hazardous substance according to osha 29 cfr 1910. Ethidium bromide laboratory spill and waste handling procedures. It is commonly abbreviated as etbr, which is also an abbreviation for bromoethane. Ac106780000, ac106780025, ac106780050, ac10678, ac106785000 synonyms. Ethidium bromide is a potent mutagen used as a nucleic acid stain. In the case of dna this is usually doublestranded dna from pcrs, restriction digests, etc. Ethidium bromide safe handling and disposal procedures. Carbon oxides, nitrogen oxides nox, hydrogen bromide gas.
Ethidium bromide cas 1239458 111615 merck millipore. Hydrogen bromide hbr safety data sheet sds p4605 praxair, inc. To avoid confusion, some laboratories have used the abbreviation ethbr. Product ethidium bromide solution, 10ml product code h5041 substance number description amount symbols h504 ethidium bromide solution 1 t.
Tufts university standard operating procedure sop for. If the pdf does not display below, you may also download it here. Sigmaaldrich 3050 spruce street saint louis mo 63103 usa telephone. For agarose gel cassettes with ethidium bromide dye dna version. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. Material safety data sheet sodium bromide 6 information in this msds is from available published sources and is believed to be accurate. Identification of the substancemixture and of the companyundertaking 1. The user must determine suitability of this information for his application.